American Society for Reproductive Medicine (ASRM) (Washington, DC）
DIFFERENCES OF THE FORMS OF HATCHING AND PREGNANCY RATES,BETWEEN LASER-ASSISTED ICSI AND NONLASER ICSI.
Laboratory, Osaka New ART Clinic, Osaka, Japan;
Doctor, Osaka New ART Clinic, Osaka, Japan.
To examine the influence on embryo development by Laserassisted ICSI.
Retrospective clinical study.
Materials and Methods
A total of 137 women seeking treatment by ICSI. The women were divided into two study groups: 84 cycles using Laser-assisted ICSI and 119 cycles using Non-laser ICSI. A compared fertilization rate, survival rate, blastocyst formation rate, pregnancy rate, implantation rate, multiple pregnancy rate, and miscarriage rate of both group.
In Laser-assisted ICSI the survival rate was higher (90.0% vs. 83.9%: P<0.001) and the hatching blastocyst formation rate was higher (26.6% vs. 14.1% : P<0.001) in comparison with Non-laser ICSI. Also, in hatching blastocyst by Laser-assisted ICSI the average embryo size was small (145.7 mm vs. 158.6 mm : P<0.05) and the zona pellucida remained thick (10.3 mm vs. 5.9 mm : P<0.0001) in comparison with Non-laser ICSI. Then, the thickness of the zona pellucida and the diameter of embryos of 28 hatching blastocysts by conventional IVF were measured. The average thickness of the zona pellucida of embryo by conventional IVF was 4.3 mm. Therefore, the thickness of the zona pellucida of Laser-assisted ICSI and Non-laser ICSI was compared based on the thickness of the zona pellucida by conventional IVF. There were fewer cases of hatching blastocysts with a rate of thickness of zona pellucida of less than 4.3 mm in Laser-assisted ICSI in comparison with Non-laser ICSI (6.7% vs. 44.4% : P<0.01). Also, the average size of the hatching blastocysts by conventional IVF was 153 mm. There were fewer cases of hatching blastocysts with a size equal to or more than 153 mm in Laser-assisted ICSI in comparison with Non-laser ICSI (20.0% vs. 55.6% : P<0.05). However, there was no difference in the pregnancy rates (31.6% vs. 30.0%), the implantation rates (14.3% vs. 14.4%), the miscarriage rates (14.2% vs. 10.7%) and the multiple pregnancy rates (0% vs. 10.7%) between Laser-assisted ICSI and Non-laser ICSI.
We found that zona pellucida thickness remained the some and average embryo size was relatively small in Laser-assisted ICSI derived hatching blastocysts. However, there were no significant differences in pregnancy rates, implantation rates, miscarriage rates and multiple pregnancy rates. Supported by: None.